Studying the adaptive evolution of Lactococcus lactis to controlled environments using a systems biology approach

Supervisor:Filipe Santos (room N229a)

We have recently developed a miniaturized continuous fermentation system suitable for prolonged cultivation. We are using this tool to study the long-term adaptation of Lactococcus lactis to various controlled environments employing a systems biology approach. L. lactis will be cultured for over 1000 generations under different conditions while monitoring changes on the genetic and transcriptional level, and its implications in flux distributions and enzyme activities.
In this project, several experimental procedures will be employed such as continuous fermentations, HPLC analysis, and sequence and transcriptome analysis, amongst others.